Direct Sequencing

A technique used to identify the sequence of individual nucleobases in a single strand of DNA fragment template, which can be a plasmid insert… (More)
National Institutes of Health

Topic mentions per year

Topic mentions per year

1989-2018
051019892018

Papers overview

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2015
2015
PURPOSE Direct sequencing (DS) is the standard method for detection of epidermal growth factor receptor (EGFR) gene mutation in… (More)
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2015
2015
The recent development of sequence-specific nuclease systems, i.e., TALENs and CRISPR/Cas9, has made genomic targeting easier in… (More)
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2013
2013
BACKGROUND The aims of this study were to evaluate the abilities of direct sequencing (DS), peptide nucleic acid (PNA) clamping… (More)
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2013
2013
Peptide nucleic acid (PNA)-mediated real-time PCR clamping has higher sensitivity than conventional direct sequencing for… (More)
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2012
2012
The objective of this study is to compare two EGFR testing methodologies (a commercial real-time PCR kit and a specific EGFR… (More)
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Highly Cited
2009
Highly Cited
2009
Understanding the key process of human mutation is important for many aspects of medical genetics and human evolution. In the… (More)
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2009
2009
BACKGROUND Increasing data from clinical trials support EGFR and K-ras mutation status as predictive markers of tumour response… (More)
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2008
2008
Direct Sanger sequencing of a diploid template containing a heterozygous insertion or deletion results in a difficult-to… (More)
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2000
2000
Preformed transposase-transposon complexes called ‘Transposomes’ have been electroporated into bacterial cells. The magnesium… (More)
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1989
1989
Mutations in codons 12 or 13 of the first exon of the N-RAS gene have been reported in myelodysplastic syndromes (MDS) in… (More)
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