Analysis of a chemical plant defense mechanism in grasses.
Five genes, Bx1 through Bx5, are required for DIBOA biosynthesis in maize and the functions of these five genes, clustered on chromosome 4, were demonstrated in vitro.
Biosynthesis of isoprenoids via the non-mevalonate pathway
- W. Eisenreich, A. Bacher, D. Arigoni, F. Rohdich
- BiologyCellular and Molecular Life Sciences (CMLS)
- 1 June 2004
The mevalonate pathway is essential in plants, many eubacteria and apicomplexan parasites, but not in archaea and animals, and detailed knowledge about the mechanisms may benefit the development of novel antibiotics, antimalarials and herbicides.
Deoxyxylulose phosphate pathway to terpenoids.
The deoxyxylulose phosphate pathway of isoprenoid biosynthesis: Studies on the mechanisms of the reactions catalyzed by IspG and IspH protein
- F. Rohdich, F. Zepeck, D. Arigoni
- Chemistry, BiologyProceedings of the National Academy of Sciences…
- 5 February 2003
A recombinant fusion protein comprising maltose binding protein and IspG protein domains was purified from a recombinant Escherichia coli strain and found that auxiliary proteins are required as shuttles for redox equivalents.
The deoxyxylulose phosphate pathway of terpenoid biosynthesis in plants and microorganisms.
Terpenoid biosynthesis from 1-deoxy-D-xylulose in higher plants by intramolecular skeletal rearrangement.
- D. Arigoni, S. Sagner, C. Latzel, W. Eisenreich, A. Bacher, M. Zenk
- ChemistryProceedings of the National Academy of Sciences…
- 30 September 1997
13C Enrichment and 13C13C coupling patterns showed conclusively that 1-deoxy-D-xylulose and not mevalonate is the predominant isoprenoid precursor of phytol, beta-carotene, and lutein.
Studies on the nonmevalonate terpene biosynthetic pathway: Metabolic role of IspH (LytB) protein
- F. Rohdich, S. Hecht, W. Eisenreich
- BiologyProceedings of the National Academy of Sciences…
- 29 January 2002
The transformation of exogenous 1-deoxy-d-xylulose into a 5:1 mixture of [U-13C5]isopentenyl diphosphate and dimethylallyl diph phosphate by an E. coli strain engineered for the expression of the ispH (lytB) gene in addition to recombinant xylB and ispCDEFG genes is described.
Studies on the nonmevalonate pathway to terpenes: The role of the GcpE (IspG) protein
- S. Hecht, W. Eisenreich, F. Rohdich
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 18 December 2001
Recombinant Escherichia coli cells engineered for the expression of the xylB gene in conjunction with genes of the nonmevalonate pathway were supplied with 13C-labeled 1-deoxy-d-xylulose. Cell…
Biosynthesis of cannabinoids
13C enrichments and coupling patterns show that the C10-terpenoid moiety is biosynthesized entirely or predominantly via the recently discovered deoxyxylulose phosphate pathway, and the phenolic moieties are generated by a polyketide-type reaction sequence.
Probing the reaction mechanism of IspH protein by x-ray structure analysis
- T. Gräwert, I. Span, M. Groll
- ChemistryProceedings of the National Academy of Sciences
- 28 December 2009
The five crystal structures of IspH in complex with substrate, converted substrate, products and PPi reported in this article provide unique insights into the mechanism of this enzyme.