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SUMMARY Escherichia coli is the predominant nonpathogenic facultative flora of the human intestine. Some E. coli strains, however… Expand High-affinity nucleic acid ligands for a protein were isolated by a procedure that depends on alternate cycles of ligand… Expand A gene expression system based on bacteriophage T7 RNA polymerase has been developed. T7 RNA polymerase is highly selective for… Expand Three kinds of improvements have been introduced into the M13-based cloning systems. (1) New Escherichia coli host strains have… Expand The RNA polymerase gene of bacteriophage T7 has been cloned into the plasmid pBR322 under the inducible control of the lambda PL… Expand A simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described. This in vitro… Expand A DNA sequence for the genome of bacteriophage φX174 of approximately 5,375 nucleotides has been determined using the rapid and… Expand Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of… Expand Escherichia coli cells of strain K12 and C can be made competent to take up temperate phage DNA without the use of “helper phage… Expand The temperate bacteriophage P1 acts as transducing agent for a large variety of genetic characteristics of strains of Escherichia… Expand