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Porphyrins have drawn much attention as sensitizers owing to the large absorption coefficients of their Soret and Q bands in the… Expand In eukaryotic cell nuclei, DNA associates with the core histones H2A, H2B, H3 and H4 to form nucleosomal core particles. DNA… Expand Biotin and avidin form one of the most stable complexes known (K(D) = 10(-15) M(-1)) making this pairing attractive for a variety… Expand Evidence is emerging that biotin participates in processes other than classical carboxylation reactions. Specifically, novel… Expand We are investigating the hypothesis that biotin multimers can be used with streptavidin and monoclonal antibody conjugates in… Expand Migration of hematopoietic precursor cells to the thymus is shown to depend upon a novel molecule called Vanin-1 expressed by… Expand Biotinidase (EC 22.214.171.124) catalyzes the hydrolysis of biocytin, the product of biotin-dependent carboxylase degradation, to… Expand Biotinidase has been purified from human serum to a specific activity of 1900 units/mg protein by a five-step procedure. After… Expand Biotinidase catalyzes the hydrolysis of N epsilon-biotinyllysine (biocytin) to form biotin and free lysine. The enzyme has been… Expand Biotinidase (EC 126.96.36.199) cleaves covalently-bound biotin from partially degraded carboxylases and from biocytin, thereby… Expand