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Discovery of a two-component monooxygenase SnoaW/SnoaL2 involved in nogalamycin biosynthesis.

It is demonstrated that inactivation of either snoaL2 or snoaW resulted in accumulation of two nonhydroxylated metabolites, nogalamycinone and a novel anthracycline 3',4'-demethoxy-nogalose-nogialamyzinone.
PubMed (opens in a new tab)

Genotyping-Guided Discovery of Persiamycin A From Sponge-Associated Halophilic Streptomonospora sp. PA3

The work demonstrates the use of multivariate data and underexplored ecological niches to guide the drug discovery process for antibiotics and anticancer agents.
PubMed (opens in a new tab)

Photosynthetically produced sucrose by immobilized Synechocystis sp. PCC 6803 drives biotransformation in E. coli

It is demonstrated that cell entrapment in Ca 2+ -alginate beads is an effective method to prolong sucrose production by the engineered cyanobacteria, while allowing efficient separation of the cells from the medium.
Springer Nature (opens in a new tab)

Laboratory course on Streptomyces genetics and secondary metabolism

The course combines genetic modification of Streptomyces; growing of the strain and protoplast preparation, plasmid isolation by alkaline lysis and phenol precipitation, digestions, and ligations prior to protoplast transformation, as well as investigating the secondary metabolites produced by the strains.
Wiley (opens in a new tab)

Divergent non-heme iron enzymes in the nogalamycin biosynthetic pathway

It is demonstrated that the proteins are evolutionarily related and that only a limited set of amino acid changes in the active sites are responsible for the switch in chemistry, encouraging for future research because it indicates that protein engineering could lead to generation of novel compounds with improved biological activities.
NAS (opens in a new tab)

Co-factor independent oxidases ncnN and actVA-3 are involved in the dimerization of benzoisochromanequinone antibiotics in naphthocyclinone and actinorhodin biosynthesis

The sequenced genome of S. arenae DSM40737 was used to identify the naphthocyclinone gene cluster and established heterologous production in S. albus J1074, suggesting that dimerization of benzoisochromanequinone polyketides require two-component reductase-oxidase systems.
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The Rieske Oxygenase SnoT Catalyzes 2′′‐Hydroxylation of l‐Rhodosamine in Nogalamycin Biosynthesis

It is demonstrated that the Rieske enzyme SnoT catalyzes 2′′‐hydroxylation of l‐rhodosamine as an initial post‐glycosylation step in nogalamycin biosynthesis, and that the reaction order continues with 2–5′′ carbocyclization and 4′′ epimerization by the non‐heme iron and 2‐oxoglutarate‐dependent enzymes SnoK and SnoN, respectively.
Wiley (opens in a new tab)

Crystal structure of the epimerase SnoN in complex with Fe3+, alpha ketoglutarate and nogalamycin RO

Publisher (opens in a new tab)

Mapping Nanocellulose- and Alginate-Based Photosynthetic Cell Factory Scaffolds: Interlinking Porosity, Wet Strength, and Gas Exchange

To develop efficient solid-state photosynthetic cell factories for sustainable chemical production, we present an interdisciplinary experimental toolbox to investigate and interlink the structure,…
PubMed (opens in a new tab)

Discovery of the Showdomycin Gene Cluster from Streptomyces showdoensis ATCC 15227 Yields Insight into the Biosynthetic Logic of C-Nucleoside Antibiotics.

Nucleoside antibiotics are a large class of pharmaceutically relevant chemical entities, which exhibit a broad spectrum of biological activities. Most nucleosides belong to the canonical N-nucleoside…
PubMed (opens in a new tab)
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