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aqualysin I

National Institutes of Health

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Serine Endopeptidases

Papers overview

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2018
2018
Expression of Aqualysin I under a Bacteriophage T 7 ( a Thermophilic Protease ) in Promoter Soluble Form in Escherichia coli
The thermophilic protease aqualysin I (AQI) gene (ae"I), derived from 11hermus aguaticus YT-1, was inserted under the contro] of… 
2016
2016
A single mutation Gln142Lys doubles the catalytic activity of VPR, a cold adapted subtilisin-like serine proteinase.
2016
2016
The effect of different rotamers of histidine-70 on the reaction rates of aqualysin I, a subtilase from the thermophilic bacterium Thermus aquaticus
Subtilisin-like serine proteases have proven to be useful as model proteins in protein engineering to better understand catalytic… 
2015
2015
Rational design of the cold active subtilisin-like serine protease VPR towards higher activity and thermostability
This research project builds on research previously done on the subtilisin-like serine proteinase VPR, from a psychrotrophic… 
2014
2014
Flexibility of cold- and heat-adapted subtilisin-like serine proteinases evaluated with fluorescence quenching and molecular dynamics.
1999
1999
Substrate Specificity of Aqualysin I Altered by an Organic Solvent, DMSO.
Aqualysin I is the alkaline serine protease isolated from an extreme thermophile, Thermus aquaticus YT-1. We have analyzed the… 
1995
1995
Expression of aqualysin I (a thermophilic protease) in soluble form in Escherichia coli under a bacteriophage T7 promoter.
The thermophilic protease aqualysin I (AQI) gene (aquI), derived from Thermus aquaticus YT-1, was inserted under the control of… 
1994
1994
Fermentation conditions for efficient production of thermophilic protease in Escherichia coli harboring a plasmid
Escherichia coli TG1, transformed with an expression plasmid pAQN carrying the aqualysin I (AQI) gene derived from Thermus… 
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