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We introduce Salmon, a lightweight method for quantifying transcript abundance from RNA–seq reads. Salmon combines a new dual… Expand MOTIVATION
Accurate alignment of high-throughput RNA-seq data is a challenging and yet unsolved problem because of the non… Expand Differential analysis of gene and transcript expression using high-throughput RNA sequencing (RNA-seq) is complicated by several… Expand Recent advances in high-throughput cDNA sequencing (RNA-seq) can reveal new genes and splice variants and quantify expression… Expand BackgroundRNA-Seq is revolutionizing the way transcript abundances are measured. A key challenge in transcript quantification… Expand High-throughput mRNA sequencing (RNA-Seq) promises simultaneous transcript discovery and abundance estimation. However, this… Expand Summary: It is expected that emerging digital gene expression (DGE) technologies will overtake microarray technologies in the… Expand Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to… Expand Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an… Expand We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate… Expand