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TM4SF4 gene

Known as: INTESTINE AND LIVER TETRASPAN MEMBRANE PROTEIN, il-TMP, ILTMP 
National Institutes of Health

Related topics

Related topics

1 relation
TM4SF18 gene

Papers overview

Semantic Scholar uses AI to extract papers important to this topic.
2019
2019
Identification of residues critical for topology inversion of the transmembrane protein TM4SF20 through regulated alternative translocation
Adopting a proper topology is crucial for transmembrane proteins to perform their functions. We previously reported that ceramide… 
Review
2018
Review
2018
GWAS : Genomewide association study HDLC : High-density lipoprotein cholesterol LD : Linkage disequilibrium LDL-C : Low-density lipoprotein cholesterol MAF : Minor allele frequency TC : Total…
This article has been accepted for publication and undergone full peer review but has not been through the copyediting… 
2017
2017
The integrated pathway of TGFβ/Snail with TNFα/NFκB may facilitate the tumor-stroma interaction in the EMT process and colorectal cancer prognosis
Substantial evidence has shown that epithelial-mesenchymal transition (EMT) plays critical roles in colorectal cancer (CRC… 
2014
2014
Interaction of TM4SF4 with the human thiamine (vitamin B1) transporter‐2 (hTHTR‐2) in human intestinal epithelial cells (896.1)
The human thiamine transporter‐2 (hTHTR‐2) is involved in intestinal absorption of thiamine. Recent studies with membrane… 
Review
2014
Review
2014
Defining microenvironment-induced transcription profiles in breast cancer liver metastases
................................................................................................................................................ 2 RÉSUMÉ ..................................................................................................................................................... 3 ABBREVIATIONS ..................................................................................................................................... 5 TABLE OF CONTENTS ........................................................................................................................... 8 LIST OF FIGURES .................................................................................................................................. 10 LIST OF TABLES .................................................................................................................................... 12 ACKNOWLEDGEMENTS ..................................................................................................................... 13 CONTRIBUTION OF AUTHORS.......................................................................................................... 15 CHAPTER 1: REVIEW OF LITERATURE ......................................................................................... 16 1.1. Classification .................................................................................................................................... 18 1.1.1. Histopathology ....................................................................................................................... 18 1.1.2. Staging ................................................................................................................................... 19 1.1.3. Grading................................................................................................................................... 20 1.1.4. Molecular subtype .................................................................................................................. 20 1.2. Breast tumorigenesis ........................................................................................................................ 23 1.3. Breast cancer metastasis ................................................................................................................... 26 1.3.1. The pre-metastatic niche ........................................................................................................ 31 1.3.2. Liver metastasis ...................................................................................................................... 33 1.4. Metastatic microenvironment ........................................................................................................... 36 1.4.1. Tumor-stroma interactions in the liver microenvironment .................................................... 37 1.5. Rationale and approach .................................................................................................................... 38 CHAPTER 2: MATERIALS AND METHODS .................................................................................... 41 2.1. Cell lines and culture conditions ...................................................................................................... 42 2.2. Antibodies ........................................................................................................................................ 42 2.3. Generation of primary tumors and liver metastases ......................................................................... 42 2.4. Hematoxylin and Eosin staining ...................................................................................................... 43 2.5. Laser capture microdissection of frozen sections ............................................................................ 43 2.6. RNA isolation, amplification, labeling, and hybridization to microarrays ...................................... 44 2.7. Data processing and statistical analysis ........................................................................................... 45 2.8. Immunohistochemistry ..................................................................................................................... 45 2.9. Protein extraction ............................................................................................................................. 46 2.10. Immunoblotting .............................................................................................................................. 46 9 CHAPTER 3: RESULTS ......................................................................................................................... 48 3.1. Generation of murine primary mammary tumors and liver metastases ........................................... 49 3.2. Capturing regions of interest using LCM ......................................................................................... 50 3.3. Quality control and gene expression analysis of microarray data .................................................... 52 3.4. Class comparisons to find differentially expressed genes ................................................................ 53 3.5. Target gene validation: Metastasis core vs. margin comparison ..................................................... 55 3.5.1. Foxm1 ....................................................................................................................................... 56 3.5.2. Dnmt1 ........................................................................................................................................ 57 3.5.3. Brms1 ........................................................................................................................................ 58 3.5.4. Ndrg1 ........................................................................................................................................ 60 3.5.5. Tm4sf4 ...................................................................................................................................... 60 3.6. Gene target validations: Primary tumor vs. liver metastases .......................................................... 60 3.6.1. Lcn2 and S100a8/S100a9 as possible markers of myeloid/granulocytic cell recruitment ........ 61 CHAPTER 4: DISCUSSION AND FUTURE DIRECTIONS .............................................................. 63 4.1. Validation of genes differentially expressed between the core and margin of liver metastases ...... 65 4.2. Class comparisons between primary tumors and liver metastases revealed an enrichment of liver function genes in the metastases ............................................................................................................. 67 4.3. Lcn2 and S100a8 are putative myeloid/granulocytic cell markers .................................................. 70 4.4. Future directions emanating from this work .................................................................................... 74 REFERENCES .......................................................................................................................................... 77 APPENDIX ................................................................................................................................................ 83 
2001
2001
Molecular cloning of a cDNA for rat TM4SF4, a homolog of human il-TMP (TM4SF4), and enhanced expression of the corresponding gene in regenerating rat liver(1).
il-TMP (also known as TM4SF4) is a human tetraspanin that is expressed in human intestine and liver. We have cloned a novel cDNA… 
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