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RPI 856 A

Known as: RPI-856 A 
 
National Institutes of Health

Papers overview

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2019
2019
Potato (Solanum tuberosum L.) is leading commercial crop its production is largely constrained by devastating disease late blight… Expand
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2018
2018
Традиционные задачи селекции картофеля – повышение урожайности и устойчивости к многочисленным патогенам и вредителям. Из них наибольший ущерб картофелеводству как в России, так и мире наносит фитофтороз, вызываемый оомицетом Phytophthora infestans. Дикие виды картофеля используются в селекции в качестве источников R генов устойчивости к патогенам. Особый интерес представляют мексиканские виды, поскольку Мексика – центр происхождения и разнообразия P. infestans и центр разнообразия видов картофеля. Дикие мексиканские виды картофеля S. bulbocastanum и S. stoloniferum являются источниками R генов устойчивости к широкому спектру рас P. infestans (Rpi-blb1, Rpi-blb2, Rpi-sto1). В последние годы эти гены были интрогрессированы в геном культурного картофеля с использованием методов цис-генетики. В то же время высока вероятность выявления генотипов с геном Rpi-sto1 (функциональный гомолог Rpi-blb1) у сортов, созданных методами традиционной селекции, поскольку уже около 40 лет селекционеры используют S. stoloniferum в качестве источника устойчивости к наиболее вредоносному вирусу картофеля – PVY. В настоящей работе проведен молекулярный скрининг 188 сортов картофеля российской селекции и стран ближнего зарубежья с ген-специфичными маркерами RB/Rpi-blb1, Rpi-sto1 и Rpi-blb2; маркерами, сцепленными с генами Rysto, Ry-fsto, детерминирующими устой чивость к PVY, и маркером митотипа gamma, ассоциированного с мужской стерильностью S. stoloniferum гибридов. Отобранные в молекулярном скрининге генотипы могут быть разделены на четыре группы: (А) 13 устойчивых к PVY сортов с диагностическими маркерами генов Rysto, Ry-fsto и со стерильным мт-типом gamma; (В) четыре сорта с мт-ти пом gamma, не обладающие маркерами R генов устойчивости, интрогрессированых от S. stoloniferum; (С) восемь генотипов, у которых были детектированы все пять ген-специфич ных маркеров гена RB/Rpi-blb1/Rpi-sto1; (D) оставшиеся 166 (86.9 %) сортов выборки, у которых не были выявлены маркеры R генов устойчивости S. stoloniferum и митотип The main objectives in potato breeding are increasing yield abilities and improving resistance to numerous pathogens and pests. Among them, the late blight caused by the Phytophthora infestans oomycete is one of the most destructive potato diseases both in Russia and worldwide. Wild relatives of cultivated potato are traditionally used in breeding as the source of valuable R genes conferring resistance to pathogens. Of particular interest are Mexican wild species because Mexico is the centre of origin and diversity of P. infestans and at the same time, it is the centre of potato species diversity. Mexican wild potato species S. bulbocastanum and S. stoloniferum are an important source of the R genes conferring broad-spec trum resistance against various isolates of P. infestans (Rpi-blb1, Rpi-blb2, Rpi-sto1). Recently these genes have been transferred into cultivated potato gene pool using the cisgene approach. At the same time there is a high probability of finding geno types with the Rpi-sto1 gene (functional homologues of Rpi-blb1) among conventionally bred varieties because for about 40 years S. stoloniferum has been used in breeding as a source of the Rysto and Ry-fsto genes of the extreme resistance to the most important viral pathogen PVY. In this study 188 potato varieties bred in Russia and in near-abroad countries were screened for the presence of six gene-specific markers of the RB/Rpi-blb1 = Rpi-sto1 and Rpi-blb2 genes conferring broad-spectrum resistance against P. infestans, and for the markers linked to the Rysto and Ry-fsto genes conferring extreme resistance to PVY. In addition, a marker for detecting male sterile mitochondrial DNA type gamma derived from S. stoloniferum was used. The genotypes selected through the molecular markers were divided into four groups: (A) 13 PVY resistant varieties carrying diagnostic markers of the Rysto, Ry-fsto genes and having sterile mt-type gamma; (B) four varieties possessing mt-type gamma and not having the markers of the R genes introgressed from S. stoloniferum; (C) eight genotypes carrying five gene-specific markers for the RB/Rpi-blb1/= Rpi-sto1; (D) the rest 166 (86.9 %) varieties not possessing any of the diagnostic markers associated with the S. stoloniferum genetic material. The sequences of the Rpi-sto1and BLB1 F/R-amplicons were identical in all the genotypes of group ‘C’ and showed respective 99 % and 100 % similarity to the corresponding fragments of the Rpi-sto1 and Rpi-blb1 genes from the GenBank database. Among the genotypes of group ‘C’ various mt-types were detected, and some of them were male fertile. 
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2017
2017
Late blight has been the most devastating potato disease worldwide. The causal agent, Phytophthora infestans, is notorious for… Expand
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2017
2017
Cultivating resistant varieties of potato is the most effective and environmentally safe method of protecting against pests and… Expand
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2015
2015
Through structure-based and directed evolution approaches, a new catalytic activity has been established on the (β/α)8 barrel… Expand
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Highly Cited
2013
Highly Cited
2013
Summary RenSeq is a NB-LRR (nucleotide binding-site leucine-rich repeat) gene-targeted, Resistance gene enrichment and sequencing… Expand
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2010
2010
The potato late blight disease, caused by the oomycete Phytophthora infestans, is a major threat for potato production worldwide… Expand
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2008
2008
For breeding potato varieties resistant to late blight, identification of resistance genes to Phytophthora infestans (Rpi genes… Expand
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Highly Cited
2008
Highly Cited
2008
Allele mining facilitates the discovery of novel resistance (R) genes that can be used in breeding programs and sheds light on… Expand
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1994
1994
Four kinds of retrovirus protease inhibitors (RPI-856 A, B, C and D) were isolated as white powder from the culture filtrate of a… Expand
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