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Primer Extension

A method to synthesize cDNA for determining the 5'-untranslated region of a specific mRNA molecule through reverse transcription reaction using… Expand
National Institutes of Health

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Highly Cited
2011
Highly Cited
2011
New regulatory roles continue to emerge for both natural and engineered noncoding RNAs, many of which have specific secondary and… Expand
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Highly Cited
2008
Highly Cited
2008
Successful protein expression, purification, and crystallization for challenging targets typically requires evaluation of a… Expand
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Highly Cited
2006
Highly Cited
2006
Selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) interrogates local backbone flexibility in RNA at single… Expand
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Highly Cited
2005
Highly Cited
2005
The reactivity of an RNA ribose hydroxyl is shown to be exquisitely sensitive to local nucleotide flexibility because a… Expand
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Highly Cited
2005
Highly Cited
2005
There has been a surge of interest in the biology of microRNAs and the technology of RNA interference. We describe a simple… Expand
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Highly Cited
2001
Highly Cited
2001
The RNA world hypothesis regarding the early evolution of life relies on the premise that some RNA sequences can catalyze RNA… Expand
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Highly Cited
1992
Highly Cited
1992
We report on the development and application of a rapid assay for detecting and typing dengue viruses. Oligonucleotide consensus… Expand
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Highly Cited
1990
Highly Cited
1990
The human progesterone receptor (hPR) cDNA, synthesized from T47D breast cancer cells, and the hPR gene 5′‐flanking region were… Expand
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Highly Cited
1988
Highly Cited
1988
Polymerase chain reaction conditions were established for the in vitro amplification of eukaryotic small subunit ribosomal (16S… Expand
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Highly Cited
1987
Highly Cited
1987
The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and… Expand
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