Primer Extension

A method to synthesize cDNA for determining the 5'-untranslated region of a specific mRNA molecule through reverse transcription reaction using… (More)
National Institutes of Health

Papers overview

Semantic Scholar uses AI to extract papers important to this topic.
Highly Cited
2011
Highly Cited
2011
New regulatory roles continue to emerge for both natural and engineered noncoding RNAs, many of which have specific secondary and… (More)
Is this relevant?
Highly Cited
2008
Highly Cited
2008
Successful protein expression, purification, and crystallization for challenging targets typically requires evaluation of a… (More)
Is this relevant?
Highly Cited
2006
Highly Cited
2006
Selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE) interrogates local backbone flexibility in RNA at single… (More)
  • figure 1
  • figure 2
  • figure 3
  • table 1
  • figure 4
Is this relevant?
Highly Cited
2005
Highly Cited
2005
The reactivity of an RNA ribose hydroxyl is shown to be exquisitely sensitive to local nucleotide flexibility because a… (More)
  • figure 1
  • figure 2
  • figure 3
  • figure 4
  • figure 6
Is this relevant?
Highly Cited
2005
Highly Cited
2005
There has been a surge of interest in the biology of microRNAs and the technology of RNA interference. We describe a simple… (More)
  • figure 1
  • table 1
  • table 1
  • figure 2
  • figure 3
Is this relevant?
Highly Cited
2001
Highly Cited
2001
The RNA world hypothesis regarding the early evolution of life relies on the premise that some RNA sequences can catalyze RNA… (More)
  • figure 1
  • figure 2
  • table 1
  • figure 3
  • table 2
Is this relevant?
Highly Cited
2000
Highly Cited
2000
At present, the cost of genotyping single nucleotide polymorphisms (SNPs) in large numbers of subjects poses a formidable problem… (More)
  • table 1
  • table 2
  • table 3
Is this relevant?
Highly Cited
1997
Highly Cited
1997
We have developed a rapid quantitative method (Ms-SNuPE) for assessing methylation differences at specific CpG sites based on… (More)
  • figure 2
  • figure 1
  • figure 3
Is this relevant?
Highly Cited
1996
Highly Cited
1996
A mutation analysis method based upon a wild-type DNA sequence is presented. Oligonucleotides were utilized for primer extension… (More)
Is this relevant?
Highly Cited
1987
Highly Cited
1987
The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and… (More)
Is this relevant?