.......................................................................................................................................... 1 1 Data Processing ....................................................................................................................... 1 1.1 Data compilation from various sources ........................................................................... 1 1.2 Normalization and synonymization ................................................................................. 2 1.3 Georeferencing................................................................................................................. 3 2 Species distribution models (SDMs) ........................................................................................ 8 2.1 SDM background .............................................................................................................. 8 2.2 Environmental variables ................................................................................................... 8 2.3 SDM construction and evaluation .................................................................................... 9 2.4 SDM Products ................................................................................................................... 9 3 Conservation area network planning .................................................................................... 10 4 Status survey.......................................................................................................................... 14 4.1 Methods ......................................................................................................................... 14 4.2 Noteworthy findings from the survey ............................................................................ 16 4.3 Summary of findings ...................................................................................................... 19 4.4 Survey addendum .......................................................................................................... 21 5 Acknowledgements ............................................................................................................... 24 6 Literature Cited ...................................................................................................................... 24 7 Suggested Citation ................................................................................................................. 25 Appendix 1. Query metadata for initial data gathering of target species occurrence data ......... 26 Appendix 2. All entities contributing data to the initial dataset and accepted codons. .............. 29 Appendix 3. Specimens catalogued into the Texas Natural History Collection, Ichthyology Collection, from the Brazos River survey done as part of this project ......................................... 40 Appendix 4. Species distribution model maps.............................................................................. 52 Appendix 5. Maxent’s jackknife test of variable importance for each species. ........................... 61 8 Supplemental Data ................................................................................................................ 70 Final report to Texas Parks and Wildlife Department contract UTA11-000958, Section 6 grant TX E-136-R, TPWD #416853 iii List of Figures Figure 1. Georeferenced occurrence records of Hybognathus amarus........................................................ 4 Figure 2. Georeferenced records of Notropis girardi ................................................................................... 5 Figure 3. Georeferenced records of Platygobio gracilis. ............................................................................... 5 Figure 4. Georeferenced records of Pteronotropis hubbsi ........................................................................... 6 Figure 5. Georeferenced records of Percina maculata. ................................................................................ 7 Figure 6. Georeferenced records of Macrhybopsis tetranema. ................................................................... 7 Figure 7. A decision-making process with indication of potential entry points for the use of SDMs in influencing conservation planning work. Adapted from Guisan et al., 2013. .............................................. 8 Figure 8. Map of priority areas required for the protection of 10 to 90 percent of the modeled habitat of the selected species. This was done to achieve targeted representation of the species in minimal area while maintaining as much connectivity as possible by minimizing clustering. ......................................... 11 Figure 9. Map of the management areas when species targets vary according to conservation status. Species with federal status of endangered had a 90% target level, federally threatened = 67%, federal candidates for listing as well as state listed species = 50%, and all other species = 20%. .......................... 12 Figure 10. Map of the management areas when the targets of representation were 20 percent. ........... 13 Figure 11. Map of the management areas when the targets of representation were 30 percent. ........... 14 Figure 12. Map of Brazos survey site distribution. The insets indicate historic distributions of N. buccula and N. oxyrhynchus with more recent collections shown in red. The box in both insets indicate the extent of the survey. .............................................................................................................................................. 15 Figure 13. Proportions of various species collected. The three individual charts include families, species within Cyprinidae, and numbers of cyprinids captured other than C. lutrensis, C. venusta, and P. vigilax. .................................................................................................................................................................... 16 Figure 14. Distribution of Cyprinodon variegatus captured during survey shown alongside records from the Fishes of Texas Project’s initial dataset of specimen-based records (track1) which documents the distribution prior to discovery of this species in the upper and middle Brazos. ........................................ 17 Figure 15. Distribution of Fundulus grandis captured during survey shown alongside records from the Fishes of Texas Project’s initial dataset of specimen-based records (track1) which documents the distribution prior to our survey. ................................................................................................................. 18 Figure 16. Distribution of Poecilia latipinna captured during survey shown alongside records from the Fishes of Texas Project’s initial dataset of specimen-based records (Track1) which documents the distribution prior to discovery of this species in the middle Brazos. .......................................................... 19 Figure 17. Patterns of non-native and cyprinid richness across survey sites in the Brazos River. Cyprinid richness values exclude records of the non-native common carp. ............................................................ 21 List of Tables Table 1. Data summary for occurrence records of the six target species (see also Figures 1-6). ................ 4 Table 2. Environmental variables used in species distribution models. ..................................................... 10 Table 3. Brazos survey site details and sampling dates. ............................................................................. 22 Table 4. Brazos survey effort summary. Total length (meters) of all seine hauls at each sample site by seine dimensions (width X height X mesh). ......................................................................................................... 23 Final report to Texas Parks and Wildlife Department contract UTA11-000958, Section 6 grant TX E-136-R, TPWD #416853