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ii Preface
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iii Table of contents
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v List of figures
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vii List of abbreviations
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viii Acknowledgements
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xi Chapter 1: Introduction
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1 1.1
Plant immunity
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1 1.2
Receptor-like kinases (RLKs) and receptor-like proteins (RLPs)
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3 1.3
Genetic analysis of defence responses regulated by BIR1 and SOBIR1
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4 1.4 ER-QC in plant immunity
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7 Chapter 2: Regulation of SOBIR1 accumulation and activation of defence responses in bir1-1 by ER-quality control components CRT3, ERdj3b and SDF2
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12 2.1 Introduction
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12 2.2 Results
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15 2.2.1 Identification and characterization of sobir3-1 bir1-1 pad4-1
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15 2.2.2 SOBIR3 encodes CALRETICULIN 3
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17 2.2.3 CRT3 is required for cell death and defence responses in bir1-1
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19 2.2.4 Identification of an allele of erdj3b that supresses bir1-1 pad4-1 mutant phenotypes
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21 2.2.5 ERdj3b is required for cell death and defence responses in bir1-1
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23 2.2.6 Identification of an allele of sdf2 that supresses bir1-1 pad4-1 mutant phenotypes
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25 2.2.7 SDF2 is required for cell death and defence responses in bir1-1
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27 2.2.8 CRT3 and ERdj3b are required for the accumulation of SOBIR1
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29 2.3 Discussion
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30 2.4 Experimental procedures
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32 2.4.1 Plant material
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32 2.4.2 Mutant characterization
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33 vi 2.4.3 Analysis of SOBIR1 protein levels in crt3-1 and erdj3b-1 mutant plants
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34 Chapter 3: Regulation of SOBIR1 accumulation and activation of defence responses in bir1-1 by ER-quality control components UGGT and STT3a
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35 3.1 Introduction
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35 3.2 Results
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37 3.2.1 Identification and characterization of sobir6-1 bir1-1 pad4-1
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37 3.2.2 SOBIR6 encodes UGGT
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38 3.2.3 UGGT is required for constitutive defence responses in bir1-1
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41 3.2.4 The autoimmune phenotype of bir1-1 is partially suppressed by stt3a-2
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42 3.2.5 Accumulation of SOBIR1 is reduced in sobir6-1 but unaffected in stt3a-2
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45 3.3 Discussion
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46 3.4 Experimental procedures
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48 3.4.1 Plant material
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48 3.4.2 Mutant characterization
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48 3.4.3 Analysis of SOBIR1 protein levels in sobir6-1 and stt3a-2 mutant plants
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49 Chapter 4: Future directions and conclusions
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50 References:
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52