Hypervariable RAPD, ISSR and SSR markers generate robust taxonomic groups among Puccinia striiformis formae speciales of importance to Australian agriculture
@article{Spackman2010HypervariableRI, title={Hypervariable RAPD, ISSR and SSR markers generate robust taxonomic groups among Puccinia striiformis formae speciales of importance to Australian agriculture}, author={Merrin Elizabeth Spackman and Francis Ogbonnaya and John Stafford Brown}, journal={Australasian Plant Pathology}, year={2010}, volume={39}, pages={226-233}, url={https://api.semanticscholar.org/CorpusID:21992049} }
Size variation in the intergenic region of rRNA and variations in the SSR RJ24 profile are recommended as diagnostic tools to distinguish P. hordei from P. tritici and BGYR in the internal transcribed spacers regions of r RNA.
17 Citations
Genetic diversity of Puccinia striiformis from cereals in Alberta, Canada
- 2014
Biology, Environmental Science
Genotypic diversity was low, population genetic analysis indicated a clonal structure, and the genotypes were widely dispersed, in both formae speciales.
Virulence and molecular analysis of atypical pathotypes of yellow rust pathogen in India
- 2019
Biology, Environmental Science
Molecular variation studies showed that new Pst pathotypes are more close to known pathotypes from barley than from wheat, and principle component analysis (PCoA) explained 62.91% variation among the analysed pathotypes.
Virulence and molecular analysis of atypical pathotypes of yellow rust pathogen in India
- 2019
Biology, Environmental Science
Molecular variation studies showed that new Pst pathotypes are more close to known pathotypes from barley than from wheat, and principle component analysis (PCoA) explained 62.91% variation among the analysed pathotypes.
Virulence and molecular diversity among Puccinia striiformis f. sp. tritici pathotypes identified in India between 2015 and 2019
- 2021
Environmental Science, Biology
The genetic basis of resistance to barley grass yellow rust (Puccinia striiformis f. sp. pseudo-hordei) in Australian barley cultivars
- 2014
Agricultural and Food Sciences, Biology
Genetic analyses of 16 Australian and three exotic barley lines indicated that each carried at least a single gene for resistance to P. striiformis, andAllelism tests between genotypes that exhibited seedling infection type responses to BGYR that were similar to Franklin and Yerong revealed that resistance in most were genetically independent of Rpsp-hFranklin and RpsP-hYerong.
Genetic Variation of Puccinia triticina Populations in Iran from 2010 to 2017 as Revealed by SSR and ISSR Markers
- 2023
Environmental Science, Biology
A significant correlation was found between SSR and ISSR genotypes and the virulence phenotypes of the isolates, as determined in a previous study.
Understanding the development and management of stripe rust in central Alberta
- 2015
Agricultural and Food Sciences, Environmental Science
Low to moderate disease pressure in central Alberta was sufficient to cause significant yield losses in susceptible spring wheat cultivars, while there was limited to no yield reduction in resistant or intermediate cultivars in this region.
Detection of molecular polymorphism of Puccinia triticina from wheat in Romania.
- 2014
Agricultural and Food Sciences, Biology
Results showed a reduced polymorphism at population level, but not identity, as indicated the RAPD analysis, and evidence for molecular variability between single urediniospores isolates will be correlated with the virulence phenotypic analysis in further studies.
Diversity of Puccinia striiformis on cereals and grasses.
- 2011
Biology, Environmental Science
This has been the case since 2000, where large-scale epidemics in warmer wheat-growing areas have been ascribed to the emergence of two closely related yellow rust strains with increased aggressiveness and tolerance to warm temperatures.
Molecular and host specificity studies of Puccinia Striiformis in Australia
- 2013
Biology, Environmental Science
806 primer pairs were designed using genomic sequence data and screened using selected Puccinia species and representative formae speciales, resulting in twenty-five polymorphic SSR markers, specific for selective and sensitive studies of stripe rust.
35 References
Virulence and polymorphic DNA relationships of Puccinia striiformis f.sp. hordei to other rusts.
- 1995
Biology, Environmental Science
Based on molecular variation, none of the three formae speciales of P. striiformis were more closely related to P. hordei, P. tritici, or P. poae (stripe rust of bluegrass) in North America.
A PCR-Based Assay for Detection of Puccinia striiformis f. sp. tritici in Wheat.
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Biology, Agricultural and Food Sciences
A specific and sensitive polymerase chain reaction (PCR) assay for detecting the pathogen in wheat (Triticum aestivum) leaves was developed and required only 0.1 pg of the target DNA for a detectable and reliable amplification with the PSF and PSR primers.
Molecular mapping as a tool for pre-emptive breeding for resistance to the exotic barley pathogen, Puccinia striiformis f. sp. hordei
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Agricultural and Food Sciences, Biology
The mapping of stripe rust resistance genes in Tallon/Kaputar (TK) and Arapiles/Franklin (AF) populations is described and the usefulness of markers associated with alternative sources of resistance previously identified in the varieties Orca and Shyri in the USA is reported on.
Support for a stepwise mutation model for pathogen evolution in Australasian Puccinia striiformis f.sp. tritici by use of molecular markers
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Biology, Environmental Science
The lack of molecular variation in the Australian and New Zealand collection is consistent with the stepwise mutation theory of pathotype evolution from a single introduction.
A new variant of Puccinia striiformis causing stripe rust on barley and wild Hordeum species in Australia
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Biology, Environmental Science
During the survey in 1998, stripe rust samples were collected from wheat and grasses (principally barley grass Hordeum spp.) and occasionally barley, however, the samples were unusual in two respects.
Relatedness Among Agronomically Important Rusts Based on Mitochondrial Cytochrome b Gene and Ribosomal ITS Sequences
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Agricultural and Food Sciences, Biology
The results demonstrate that the amino acid sequence of the mitochondrial cytochrome b is a valid tool to study phylogenic relatedness among plant pathogenic Basidiomycetes and supports taxonomic grouping based on morphological structures and host specificity.
Detection of Polymorphism in Puccinia hordei using RFLP and RAPD Markers, Differential Cultivars, and Analysis of the Intergenic Spacer Region of rDNA
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Biology, Environmental Science
A world-wide range of isolates of Puccinia hordei and P. striiformis f. sp. hordei and UK isolates of P. striiformis f. sp. tritici and P. recondita were characterized using DNA restriction fragment…
Molecular genetic variability of Australian isolates of five cereal rust pathogens.
- 2003
Biology, Environmental Science
Of the three marker types, SAMS were the most informative, and have the potential for the development of locus-specific microsatellites, and the major differences were consistent and polymorphism was generally low.
Puccinia striiformis in Australia: a review of the incursion, evolution, and adaptation of stripe rust in the period 1979–2006
- 2007
Environmental Science, Biology
Data is suggested that BGYR is a new forma specialis of P. striiformis, likely to have little impact on commercial barley, although this may change with further pathotype evolution or the release of susceptible cultivars.
Detection and identification of four common rust pathogens of cereals and grasses using real-time polymerase chain reaction.
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Agricultural and Food Sciences, Biology
Real-time polymerase chain reaction assays for rapid detection of the four rust pathogen species, Puccinia graminis, P. striiformis, Triticina, triticina and recondita were developed and validating the integrity of each assay was validated.