Genomic and Transcriptome Analysis Reveals the Biosynthesis Network of Cordycepin in Cordyceps militaris

@article{Chai2024GenomicAT,
  title={Genomic and Transcriptome Analysis Reveals the Biosynthesis Network of Cordycepin in Cordyceps militaris},
  author={Linshan Chai and Jianmei Li and Lingling Guo and Shuyu Zhang and Fei Chen and Wanqin Zhu and Yu Li},
  journal={Genes},
  year={2024},
  volume={15},
  url={https://api.semanticscholar.org/CorpusID:269871813}
}
Cordycepin is the primary active compound of Cordyceps militaris. However, the definitive genetic mechanism governing cordycepin synthesis in fruiting body growth and development remains elusive, necessitating further investigation. This study consists of 64 C. militaris strains collected from northeast China. The high-yielding cordycepin strain CMS19 was selected for the analysis of cordycepin production and the genetic basis of cordycepin anabolism. First, the whole-genome sequencing of CMS19… 
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64 References

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