toluene dioxygenase activity

Known as: toluene 1,2-dioxygenase activity, toluene 2,3-dioxygenase activity, toluene,NADH:oxygen oxidoreductase (1,2-hydroxylating)

Catalysis of the reaction: H(+) + NADH + O(2) + toluene = (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD(+). [EC:1.14.12.11, RHEA:16740]

National Institutes of Health

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1987

Toluene was oxidized by a mutant strain of Pseudomonas putida (strain NG1) to toluene Cis‐Glycol (TCG). Product was accumulated…