mRNA Cleavage and Polyadenylation Factors
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Purpose: Alternative cleavage and polyadenylation (APA) of mRNAs is a phenomenon that alters 3′-untranslated region length… Expand HIV-1 replication requires transport of nascent viral DNA and associated virion proteins, the retroviral preintegration complex… Expand Messenger RNA 3′-end formation is functionally coupled to transcription by RNA polymerase II. By tagging and purifying Ref2, a… Expand Set1p methylates lysine 4 (K4) of histone H3 and regulates the expression of many genes in yeast. Here we use a biochemical… Expand Cleavage and polyadenylation factor (CPF) is a multi-protein complex that functions in pre-mRNA 3'-end formation and in the RNA… Expand Recognition of poly(A) sites in yeast pre‐mRNAs is poorly understood. Employing an in vitro cleavage system with cleavage and… Expand We have examined the distribution of RNA transcription and processing factors in the amphibian oocyte nucleus or germinal vesicle… Expand Cleavage and polyadenylation factor I (CF I) is one of four factors required in vitro for yeast pre-mRNA 3'-end processing. Two… Expand Cleavage and polyadenylation factor (CPF) is required for the cleavage as well as for the subsequent polyadenylation reaction… Expand Polyadenylation of messenger RNA precursors requires the nucleotide sequence AAUAAA and two factors: poly(A) polymerase and a… Expand