..............................................................................................................................................1 Introduction ......................................................................................................................................1 Muscle
development
in
domesticated
mammals............................................................................1 IGFs
in
muscle
development ..................................................................................................................2 Gene
Regulation .........................................................................................................................................3 Regulatory
Mutation
in
IGF2 ..................................................................................................................3 ZBED6
controlling
IGF2
expression .....................................................................................................5 Long
non-coding
RNAs .............................................................................................................................6 Transcription
Factors
and
Biological
Databases ............................................................................7 Materials
and
Methods ..................................................................................................................8 Chromatin
Immuno
precipitation
(ChIP)
data
(ZBED6
target
genes)......................................8 Microarray
data
(expression
of
ZBED6-silenced
genes) ..............................................................8 TRANSFAC.....................................................................................................................................................8 MatchTM .......................................................................................................................................................8 Dataset
used
For
MatchTM .....................................................................................................................9 Long
Non-coding
RNA............................................................................................................................ 10 Results.............................................................................................................................................. 12 ChIP
Datasets............................................................................................................................................ 12 Functional
Analysis
of
ZBED6...........................................................................................................................12 Transcription
factors
in
Promoter
region
of
ZBED6
target
gene ......................................................12 Microarray
Datasets............................................................................................................................... 16 Putative
Transcription
Factors
in
ZBED6-‐silenced
Microarray
data...............................................16 Long
Non-Coding
RNA............................................................................................................................ 18 Discussion....................................................................................................................................... 20 ChIP
experiment...................................................................................................................................... 20 TRANSFAC:
MatchTM............................................................................................................................. 21 Transcription
factors
in
ZBED6
target
genes
promoters .......................................................... 21 Long
non-coding
RNA ............................................................................................................................ 22 Transcription
factors
in
differentially
expressed
genes
in
ZBED6-silenced
C2C12
cells ....................................................................................................................................................................... 23 Conclusion ...................................................................................................................................... 25 Future
Prospects .......................................................................................................................... 25 Acknowledgments........................................................................................................................ 25 References ...................................................................................................................................... 26