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Real-Time Polymerase Chain Reaction

Known as: Real Time-PCR, Quantitative Real Time PCR, PCR, Real-Time 
An application of PCR that measures the products generated during each cycle of the polymerase chain reaction process in order to determine the… Expand
National Institutes of Health

Papers overview

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Highly Cited
2013
Highly Cited
2013
BACKGROUND The 2-ΔΔCT method has been extensively used as a relative quantification strategy for quantitative real-time… Expand
Highly Cited
2010
Highly Cited
2010
Soils collected across a long-term liming experiment (pH 4.0–8.3), in which variation in factors other than pH have been… Expand
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Highly Cited
2010
Highly Cited
2010
Background Recent evidence suggests that there is a link between metabolic diseases and bacterial populations in the gut. The aim… Expand
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Highly Cited
2009
Highly Cited
2009
Despite the central role of quantitative PCR (qPCR) in the quantification of mRNA transcripts, most analyses of qPCR data are… Expand
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Highly Cited
2008
Highly Cited
2008
A decrease in the abundance and biodiversity of intestinal bacteria within the dominant phylum Firmicutes has been observed… Expand
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Highly Cited
2006
Highly Cited
2006
The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data… Expand
Highly Cited
2005
Highly Cited
2005
Quantitative real-time polymerase chain reactions (qRT-PCR) have become the method of choice for rapid, sensitive, quantitative… Expand
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Highly Cited
2005
Highly Cited
2005
Real-time polymerase chain reaction (PCR) is a highly sensitive method that can be used for the detection and quantification of… Expand
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Highly Cited
2001
Highly Cited
2001
  • M. Pfaffl
  • Nucleic acids research
  • 2001
  • Corpus ID: 9003591
Use of the real-time polymerase chain reaction (PCR) to amplify cDNA products reverse transcribed from mRNA is on the way to… Expand
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Highly Cited
1996
Highly Cited
1996
A novel approach to quantitative reverse transcriptase polymerase chain reaction (QC RT-PCR) using real time detection and the 5… Expand
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