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Real-time QPCR based methods for determination of plasmid copy number in recombinant Escherichia coli cultures are presented. Two… Expand From Mycobacterium avium species Mycobacterium avium subsp. paratuberculosis (n=961), Mycobacterium a. avium (n=677… Expand A versatile plasmid vector was designed to direct the synthesis of recombinant proteins in either one of two forms that will be… Expand An Azotobacter vinelandii mannuronan C-5-epimerase gene was cloned in Escherichia coli. This enzyme catalyzes the Ca(2… Expand We have developed a simple procedure for generating mutants of the cyanobacterium Synechococcus sp. strain PCC 7942 in which the… Expand P6, a 16,600-dalton protein present in the outer membranes of both typeable and nontypeable strains, may be an important antigen… Expand A new strategy for molecular cloning in the cyanobacterium Anacystis nidulans R-2 is described. This strategy involved the use of… Expand Seven oligonucleotide primers complementary to the plasmid vector pBR322 at positions adjacent to five of the unique restriction… Expand The genome of the human polyomavirus JC (Mad-1 strain) was molecularly cloned in Escherichia coli by using the plasmid vector… Expand Abstract The sequence organization of the 1.688 satellite DNA (density 1.688 g/cm 3 in CsCl) has been investigated, and this… Expand