..........................................................................................................................................2 Introduction .....................................................................................................................................4 Methods Part I: Characterization of the immune response following personalized neoantigen-based vaccination .........................................................................................................................11 Part II: Identification of neoantigen-reactive T cell receptors and their cognate antigens using a streamlined cloning and expression system ..........................................................15 Results Part I: Characterization of the immune response following personalized neoantigen-based vaccination ........................................................................................................................19 Part II: Identification of neoantigen-reactive T cell receptors and their cognate antigens using a streamlined cloning and expression system .........................................................28 Discussion .....................................................................................................................................34 Conclusions ...................................................................................................................................42 Suggestions for Future Work ........................................................................................................43 Lay Summary ................................................................................................................................46 References .....................................................................................................................................47 Supplement ...................................................................................................................................53