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Freeze Substitution

Known as: Substitution, Freeze 
A modification of the freeze-drying method in which the ice within the frozen tissue is replaced by alcohol or other solvent at a very low… Expand
National Institutes of Health

Papers overview

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Highly Cited
2008
Highly Cited
2008
The emergence of electron tomography as a tool for three dimensional structure determination of cells and tissues has brought its… Expand
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Highly Cited
1996
Highly Cited
1996
Sensitive and high-resolution immunocytochemical procedures were used to investigate the spatial organization of AMPA receptor… Expand
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Highly Cited
1995
Highly Cited
1995
The glutamate transporters GLT-1 and GLAST were studied by immunogold labeling on ultrathin sections of rat brain tissue embedded… Expand
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Highly Cited
1994
Highly Cited
1994
Spores of Gigaspora margarita Becker &Hall, an arbuscular mycorrhizal fungus, were cryofixed using high-pressure freezing and… Expand
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Highly Cited
1993
Highly Cited
1993
As compared to classical chemical fixation, the physical immobilization of ultrastructures by fast‐freeze fixation (FFF) and the… Expand
1991
1991
Cryofixation and freeze substitution methods were developed for ultrastructural studies of cells in complex plant tissues. Leaf… Expand
Highly Cited
1991
Highly Cited
1991
The objective of the current research was to examine the response of woody plant tissues to freezing stress by using scanning… Expand
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1986
1986
The freeze substitution histological tech­ nique allows the indirect light microscopicobser­ vation and the quantitative… Expand
Highly Cited
1980
Highly Cited
1980
Antennae of the silk moth, Bombyx mori, were frozen by immersion into propane at -180 degrees C, and further processed by (a… Expand
1969
1969
The loss of 14C ethanolamine‐ and 3H choline‐labelled phospholipids from rat liver during tissue preparation for electron… Expand