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Freeze Substitution

Known as: Substitution, Freeze 
A modification of the freeze-drying method in which the ice within the frozen tissue is replaced by alcohol or other solvent at a very low… Expand
National Institutes of Health

Papers overview

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Highly Cited
2008
Highly Cited
2008
The emergence of electron tomography as a tool for three dimensional structure determination of cells and tissues has brought its… Expand
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Highly Cited
2005
Highly Cited
2005
SummaryTo circumvent the limitations of chemical fixation (CF) and to gain more reliable structural information about higher… Expand
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Highly Cited
2004
Highly Cited
2004
SummaryUsing low-temperature postembedding techniques for immunoelectron microscopy, we succeeded in demonstrating the precise… Expand
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Highly Cited
1996
Highly Cited
1996
Sensitive and high-resolution immunocytochemical procedures were used to investigate the spatial organization of AMPA receptor… Expand
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Highly Cited
1995
Highly Cited
1995
The glutamate transporters GLT-1 and GLAST were studied by immunogold labeling on ultrathin sections of rat brain tissue embedded… Expand
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Highly Cited
1994
Highly Cited
1994
summary Spores of Gigaspora margarita Becker &Hall, an arbuscular mycorrhizal fungus, were cryofixed using high-pressure… Expand
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Highly Cited
1993
Highly Cited
1993
As compared to classical chemical fixation, the physical immobilization of ultrastructures by fast‐freeze fixation (FFF) and the… Expand
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1991
1991
Cryofixation and freeze substitution methods were developed for ultrastructural studies of cells in complex plant tissues. Leaf… Expand
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Highly Cited
1980
Highly Cited
1980
Antennae of the silk moth, Bombyx mori, were frozen by immersion into propane at -180 degrees C, and further processed by (a… Expand
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1969
1969
The loss of 14C ethanolamine‐ and 3H choline‐labelled phospholipids from rat liver during tissue preparation for electron… Expand
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