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Deletion Mutagenesis

Mutagenesis where the mutation is caused by the loss of DNA sequences within a gene. This may occur spontaneously in vivo or be experimentally… 
National Institutes of Health

Papers overview

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2002
2002
The Role of the Factor X Activation Peptide: A Deletion Mutagenesis Approach
Summary To understand the role of the factor X (fX) activation peptide (AP), a deletion mutagenesis approach was employed. Two… 
1999
1999
Defining the Location and Function of Domains of McrB by Deletion Mutagenesis
Abstract The GTP-dependent restriction endonuclease McrBC of E. coli K12, which recognizes cytosine-methylated DNA, consists of… 
1996
1996
Human platelet cGI-PDE: expression in yeast and localization of the catalytic domain by deletion mutagenesis.
Cyclic adenosine monophosphate (cAMP) is an important modulator of platelet responses to agonists. Cyclic nucleotide… 
1996
1996
Identification of a locus involved in meningococcal lipopolysaccharide biosynthesis by deletion mutagenesis
A novel method for insertion/deletion mutagenesis in meningococci was devised. This consisted of ligating a digest of total… 
Highly Cited
1995
Highly Cited
1995
Selectable insertion and deletion mutagenesis of the human cytomegalovirus genome using the Escherichia coli guanosine phosphoribosyl transferase (gpt) gene.
We describe the mutagenesis of the IRSI-US5 region of the human cytomegalovirus genome, demonstrating the potential of the E… 
1992
1992
The adsorption protein of phage IKe. Localization by deletion mutagenesis of domains involved in infectivity
We constructed a set of plasmid‐encoded internal deletion mutants within the gene for the adsorption protein (g3p) of phage IKe… 
1992
1992
A method for sequence-specific deletion mutagenesis.
We describe a novel procedure for the construction of deletion mutants. Existing exonuclease-based protocols are efficient at… 
1990
1990
An analysis of the genes encoding the 51.4- and 41.9-kDa toxins of Bacillus sphaericus 2297 by deletion mutagenesis: the construction of fusion proteins.
A series of deletion mutants have been constructed, in which varying numbers of amino acids have been deleted from both the N… 
Highly Cited
1989
Highly Cited
1989
Linker insertion-deletion mutagenesis of the v-src gene: isolation of host- and temperature-dependent mutants
The host cell regulators and substrates of the Rous sarcoma virus transforming protein pp60v-src remain largely unknown. Viral… 
Highly Cited
1985
Highly Cited
1985
Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli
A mutation of a cloned gene that has been made by introducing a transposon or some other selectable genetic determinant can be… 
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