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Cell Culture Procedure

Any laboratory procedure for growing cells or tissues in vitro.
National Institutes of Health

Papers overview

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2011
2011
The Significance Application of Indigenous Phytohemagglutinin (PHA) Mitogen on Metaphase and Cell Culture Procedure
Phytohemagglutinin (PHA) is a lectin, obtained from the red kidney bean that binds to the membranes of T-cells and stimulates… Expand
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2010
2010
A role for AMPK in increased insulin action after serum starvation.
Serum starvation is a common cell culture procedure for increasing cellular response to insulin, though the mechanism for the… Expand
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Highly Cited
2008
Highly Cited
2008
Metabolic network fluxes in heterotrophic Arabidopsis cells: stability of the flux distribution under different oxygenation conditions.
Steady-state labeling experiments with [1-(13)C]Glc were used to measure multiple metabolic fluxes through the pathways of… Expand
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Highly Cited
2008
Highly Cited
2008
Mechanical and biological properties of hydroxyapatite/tricalcium phosphate scaffolds coated with poly(lactic-co-glycolic acid).
Regeneration of bone, cartilage and osteochondral tissues by tissue engineering has attracted intense attention due to its… Expand
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Highly Cited
2006
Highly Cited
2006
Confluent monolayers of cultured human fetal retinal pigment epithelium exhibit morphology and physiology of native tissue.
PURPOSE Provide a reproducible method for culturing confluent monolayers of hfRPE cells that exhibit morphology, physiology… Expand
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2006
2006
Culture of Human Skeletal Muscle Myoblasts: Timing Appearance and Localization of Dystrophin-Glycoprotein Complex and Vinculin-Talin-Integrin Complex
The dystrophin-glycoprotein complex together with the vinculin-talin-integrin complex plays an important role in muscle function… Expand
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2005
2005
Diagnosis of genitalChlamydia trachomatis infection in males by cell culture and antigen detection test
UrethralChlamydia trachomatisinfection was diagnosed in 204 of 1,011 (20.2 %) male patients by cell culture, in 219 (21.7 %) by… Expand
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Highly Cited
2004
Highly Cited
2004
Autologous human serum for cell culture avoids the implantation of cardioverter-defibrillators in cellular cardiomyoplasty.
BACKGROUND Current clinical experience with cellular cardiomyoplasty (using serum bovine-cultivated myoblasts) has demonstrated… Expand
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1999
1999
In situ and in vitro identification and characterization of cardiac ganglion neurons in the crab, Carcinus maenas.
The aim of this study was to investigate the intrinsic membrane properties and hormonal responses of individual central pattern… Expand
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1987
1987
Evaluation of enzyme immunoassay (Chlamydiazyme) for detecting Chlamydia trachomatis in genital tract specimens.
An enzyme immunoassay (Chlamydiazyme) for detecting Chlamydia trachomatis was evaluated on genital specimens from 96 men and 272… Expand
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