Bisulfite Sequencing

A DNA sequencing technique that can differentiate cytosine from 5-methylcytosine in a DNA sample. First, a denatured DNA sample is treated with… (More)
National Institutes of Health

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Highly Cited
2014
Highly Cited
2014
Single-Cell Genome-Wide Bisulfite Sequencing for Assessing Epigenetic Heterogeneity
We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48… (More)
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Highly Cited
2011
Highly Cited
2011
Preparation of reduced representation bisulfite sequencing libraries for genome-scale DNA methylation profiling
Genome-wide mapping of 5-methylcytosine is of broad interest to many fields of biology and medicine. A variety of methods have… (More)
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Highly Cited
2010
Highly Cited
2010
The Behaviour of 5-Hydroxymethylcytosine in Bisulfite Sequencing
BACKGROUND We recently showed that enzymes of the TET family convert 5-mC to 5-hydroxymethylcytosine (5-hmC) in DNA. 5-hmC is… (More)
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Highly Cited
2009
Highly Cited
2009
Targeted bisulfite sequencing reveals changes in DNA methylation associated with nuclear reprogramming
Current DNA methylation assays are limited in the flexibility and efficiency of characterizing a large number of genomic targets… (More)
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Highly Cited
2009
Highly Cited
2009
BSMAP: whole genome bisulfite sequence MAPping program
Bisulfite sequencing is a powerful technique to study DNA cytosine methylation. Bisulfite treatment followed by PCR amplification… (More)
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Highly Cited
2009
Highly Cited
2009
BS Seeker: precise mapping for bisulfite sequencing
Bisulfite sequencing using next generation sequencers yields genome-wide measurements of DNA methylation at single nucleotide… (More)
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Highly Cited
2009
Highly Cited
2009
High-throughput bisulfite sequencing in mammalian genomes.
DNA methylation is a critical epigenetic mark that is essential for mammalian development and aberrant in many diseases including… (More)
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Highly Cited
2008
Highly Cited
2008
RNA cytosine methylation analysis by bisulfite sequencing
Covalent modifications of nucleic acids play an important role in regulating their functions. Among these modifications… (More)
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Highly Cited
2005
Highly Cited
2005
Reduced representation bisulfite sequencing for comparative high-resolution DNA methylation analysis
We describe a large-scale random approach termed reduced representation bisulfite sequencing (RRBS) for analyzing and comparing… (More)
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Highly Cited
2005
Highly Cited
2005
BiQ Analyzer: visualization and quality control for DNA methylation data from bisulfite sequencing
SUMMARY Manual processing of DNA methylation data from bisulfite sequencing is a tedious and error-prone task. Here we present an… (More)
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