zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly

@article{Kuge1993zetaCOPAS,
  title={zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly},
  author={Osamu Kuge and S Hara-Kuge and Lelio Orci and Mariella Ravazzola and Myl{\`e}ne Amherdt and Garry Tanigawa and Felix Theodor Wieland and James E Rothman},
  journal={The Journal of Cell Biology},
  year={1993},
  volume={123},
  pages={1727 - 1734}
}
cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon-COP), zeta-COP exists in both coatomer bound and free pools.