xol-1: A gene that controls the male modes of both sex determination and X chromosome dosage compensation in C. elegans

@article{Miller1988xol1,
  title={
 xol-1: A gene that controls the male modes of both sex determination and X chromosome dosage compensation in C. elegans},
  author={L. M. Miller and J. Plenefisch and L. P. Casson and B. Meyer},
  journal={Cell},
  year={1988},
  volume={55},
  pages={167-183}
}
Loss-of-function mutations in the X-linked gene xol-1 cause the feminization and death of XO animals (normally males) by shifting the sex determination and dosage compensation pathways toward their hermaphrodite modes. XO-specific lethality most likely results from the reduction in X chromosome expression caused by xol-1 mutations. Mutations in genes required for the hermaphrodite mode of dosage compensation suppress lethality but not feminization, and restore X chromosome expression to nearly… Expand
xo1-1 acts as an early switch in the C. elegans male/hermaphrodite decision
TLDR
It is suggested that assessment of the X/A ratio occurs only early in embryogenesis to determine sex and that sdc-2, a gene that must be repressed by xol-1 to ensure male development, may be a direct target of negative regulation by xOL-1. Expand
Recruitment of C. elegans dosage compensation proteins for gene-specific versus chromosome-wide repression
TLDR
Dpy-21 mutations, shown here to be null, cause elevated X-linked gene expression in XX animals, but unlike mutations in other dosage compensation genes, they do not cause extensive XX-specific lethality or disrupt the stability or targeting of the dosage compensation complex to X. Expand
The role of sdc-1 in the sex determination and dosage compensation decisions in Caenorhabditis elegans.
TLDR
Analysis of 14 new sdc-1 alleles suggests that the phenotypes resulting from the lack of sDC-1 function are an incompletely penetrant sexual transformation of XX animals toward the male fate, and increased levels of X-linked gene transcripts in XX animals, correlated with XX-specific morphological defects but not significant XX- specific lethality. Expand
Untangling the Contributions of Sex-Specific Gene Regulation and X-Chromosome Dosage to Sex-Biased Gene Expression in Caenorhabditis elegans
TLDR
It is suggested that lack of dosage compensation in different tissues and developmental stages allow X chromosome copy number to contribute to sex-biased gene expression and function. Expand
The primary sex determination signal of Caenorhabditis elegans.
TLDR
It is shown that both mechanisms of xol-1 repression are essential and act synergistically to keep xol -1 levels low in XX animals, and evidence suggesting that xol, the sex-determination and dosage-compensation switch gene, can be set at intermediate levels in response to an intermediate X signal is presented. Expand
Differential effects of Sex-lethal mutations on dosage compensation early in Drosophila development.
TLDR
Results reported here suggest that the dosage compensation regulatory genes currently known to function downstream of Sxl, genes known as the "male-specific lethals," do not control all aspects of dosage compensation either at the blastoderm stage or later in development. Expand
A novel regulatory mutation in the C. elegans sex determination gene tra-2 defines a candidate ligand/receptor interaction site.
TLDR
P phenotypic characterisation of sexually transformed XO tra-2(eg) hermaphrodites reveals that their fertility is strongly affected by dosage compensation mutations, suggesting that dosage compensation plays a role in normal gametogenesis. Expand
X-Chromosome dosage compensation.
  • B. Meyer
  • Biology, Medicine
  • WormBook : the online review of C. elegans biology
  • 2005
TLDR
The dosage compensation complex resembles the conserved 13S condensin complex required for both mitotic and meiotic chromosome resolution and condensation, implying the recruitment of ancient proteins to the new task of regulating gene expression. Expand
X-chromosome-counting mechanisms that determine nematode sex
TLDR
It is demonstrated that the dose-sensitive signal elements on chromosome X control xol-1 through two different molecular mechanisms, one of which involves the transcriptional repression of xl-1 in XX animals and the other uses the putative RNA-binding protein encoded by fox-1 to reduce the level of xoli-1 protein. Expand
DPY-27: A chromosome condensation protein homolog that regulates C. elegans dosage compensation through association with the X chromosome
TLDR
It is proposed that DPY-27 implements dosage compensation by condensing the chromatin structure of X in a manner that causes general reduction of X chromosome expression. Expand
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References

SHOWING 1-10 OF 37 REFERENCES
sdc-1: A link between sex determination and dosage compensation in C. elegans
TLDR
A model in which the wild-type sdc-1 activity is either a component of the primary sex-determining signal (the X/Autosome ratio) or involved in transmitting information about this signal to both the sex determination and dosage compensation pathways is proposed. Expand
The interaction between daughterless and sex-lethal in triploids: a lethal sex-transforming maternal effect linking sex determination and dosage compensation in Drosophila melanogaster.
  • T. Cline
  • Biology, Medicine
  • Developmental biology
  • 1983
TLDR
The phenotype of triploid intersexes is shown here to survive effects of da and Sxl mutations that would be lethal to diploids. Expand
An autosomal gene that affects X chromosome expression and sex determination in Caenorhabditis elegans.
TLDR
It is proposed that dpy-21 regulates X chromosome expression and may be involved in interpreting X chromosome dose for the developmental decisions of both sex determination and dosage compensation. Expand
A genetic analysis of the sex-determining gene, tra-1, in the nematode Caenorhabditis elegans.
  • J. Hodgkin
  • Biology, Medicine
  • Genes & development
  • 1987
TLDR
The results suggest that the tra-1 gene has several roles in wild-type sexual development, and it is suggested that the function and the regulation of this gene must be complex. Expand
Activity of the sex-determining gene tra-2 is modulated to allow spermatogenesis in the C. elegans hermaphrodite.
In the nematode C. elegans, there are two sexes, the self-fertilizing hermaphrodite (XX) and the male (XO). The hermaphrodite is essentially a female that makes sperm for a brief period beforeExpand
Assessment of X chromosome dosage compensation in Caenorhabditis elegans by phenotypic analysis of lin-14.
TLDR
It is demonstrated that in diploid animals the lin-14 gene is dosage compensated, implying that the normal dosage compensation mechanism in C. elegans lacks the capacity to compensate completely for the additional X chromosome in triplo-X animals. Expand
Autoregulatory functioning of a Drosophila gene product that establish es and maintains the sexually determined state.
TLDR
This study supports the view that sex determination is truly determinative in the standard developmental sense, and that Sxl is the carrier of the sexually determined state. Expand
Dosage Compensation in Drosophila: Evidence That daughterless and Sex-lethal Control X Chromosome Activity at the Blastoderm Stage of Embryogenesis.
TLDR
The experiments indicate that dosage compensation is established early in development and demonstrate that the daughterless and Sex-lethal gene products are involved in regulating X chromosome activity at the blastoderm stage of embryogenesis. Expand
Primary sex determination in the nematode C. elegans.
TLDR
Most nematodes have XO male/XX female sex determination; C. elegans is anomalous, having XX hermaphrodites rather than females; interactions between dosage compensation and sex determination are described and discussed. Expand
Two types of sex determination in a nematode
TLDR
It is shown that stable mutant strains can be constructed in which sex is determined not by X-chromosome dosage but by the presence or absence of a single active gene. Expand
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