v-SNARE Actions during Ca2+-Triggered Exocytosis

  title={v-SNARE Actions during Ca2+-Triggered Exocytosis},
  author={Jaideep C. Kesavan and Maria Borisovska and Dieter Bruns},
Assembly of SNARE proteins between opposing membranes mediates fusion of synthetic liposomes, but it is unknown whether SNAREs act during exocytosis at the moment of Ca(2+) increase, providing the molecular force for fusion of secretory vesicles. Here, we show that execution of pre- and postfusional steps during chromaffin granule exocytosis depends crucially on a short molecular distance between the complex-forming SNARE motif and the transmembrane anchor of the vesicular SNARE protein… CONTINUE READING