trans-spliced Caenorhabditis elegans mRNAs retain trimethylguanosine caps.

  title={trans-spliced Caenorhabditis elegans mRNAs retain trimethylguanosine caps.},
  author={R. F. Liou and Thomas Blumenthal},
  journal={Molecular and cellular biology},
  volume={10 4},
The nematode Caenorhabditis elegans has an unusual small nuclear RNA, containing a 100-nucleotide RNA molecule, spliced leader RNA, which donates its 5' 22 nucleotides to a variety of recipient RNAs by a trans-splicing reaction. The spliced leader RNA has a 5' trimethylguanosine (TMG) cap, which becomes the 5' end of trans-spliced mRNAs. We found that mature trans-spliced mRNAs were immunoprecipitable with anti-TMG cap antibodies and that TMG-containing dinucleotides specifically competed with… CONTINUE READING