thermodynamic and kinetic characteristics of an α-amylase from Bacillus licheniformis SKB 4

@inproceedings{Samanta2015thermodynamicAK,
  title={thermodynamic and kinetic characteristics of an α-amylase from Bacillus licheniformis SKB 4},
  author={Saptadip Samanta and Arpan Das and Suman Kumar Halder and Arijit Jana and Sanjay Kar and Pradeep Kumar Das Mohapatra and Bikash Ranjan Pati and Keshab Chandra Mondal},
  year={2015}
}
An amylolytic bacterial strain, Bacillus licheniformis SKB4 produced maximum amylase at pH 6.5 at 42 °C, and at late stationary phase (24 h) of growth. Starch and peptone were found the best supporting carbon and nitrogen source with C:N ratio of 1:2 for amylase production. The purified enzyme was non-responsive to most of the metal ions except K+ and Mg++ (1.0 mM). The enzyme was stable and active at pH 6.5. The enzyme showed optimum temperature at 90 °C with 10 min of half life (t1⁄2) at 100… CONTINUE READING

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