tRNA punctuation model of RNA processing in human mitochondria

  title={tRNA punctuation model of RNA processing in human mitochondria},
  author={Deanna Ojala and Julio Montoya and Giuseppe Attardi},
A 3′-end proximal segment of most of the putative mRNAs encoded in the heavy strand of HeLa cell mtDNA has been partially sequenced and aligned with the DNA sequence. In all cases, the 3′-end nucleotide of the individual mRNA coding sequences has been found to be immediately contiguous to a tRNA gene or another mRNA coding sequence. These and previous results indicate that the heavy (H) strand sequences coding for the rRNA, poly (A)-containing RNA and tRNA species form a continuum extending… 

Mitochondrial poly(A) polymerase is involved in tRNA repair

It is shown that the mitochondrial poly(A) polymerase (mtPAP) is responsible for this A addition to the 3′ end of the truncated tRNA, and an enzyme machinery that evolved primarily for other purposes allows to tolerate the frequent evolutionary occurrence of gene overlaps.

RNA processing in human mitochondria

A deep sequencing approach is used to characterize the 5' and 3' ends of processed mitochondrial transcripts and provide a detailed map of mitochondrial tRNA processing sites affected by these proteins.

Structural basis of RNA processing by human mitochondrial RNase P

The cryo-EM structure of human mtRNase P bound to precursor tRNA is presented, which reveals a unique mechanism of substrate recognition and processing and provides the molecular basis for the first step of RNA processing in human mitochondria.

Transfer RNA editing in land snail mitochondria.

  • S. YokoboriS. Pääbo
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1995
It is suggested that this form of RNA editing may involve polyadenylylation of the maturing tRNAs as an intermediate in order to restore base-pairing in the stems of land snails.



The putative mRNA for subunit II of human cytochrome c oxidase starts directly at the translation initiator codon

This work sequenced a 5′-end proximal segment of the putative COII mRNA from HeLa cells of about 30 nucleotides and aligned it with the COII coding sequence in human mtDNA, showing that this RNA starts directly at the AUG initiator codon.

DNA sequence of a T4 transfer RNA gene cluster.

Sequence and organization of the human mitochondrial genome

The complete sequence of the 16,569-base pair human mitochondrial genome is presented and shows extreme economy in that the genes have none or only a few noncoding bases between them, and in many cases the termination codons are not coded in the DNA but are created post-transcriptionally by polyadenylation of the mRNAs.

Ribonuclease P substrate specificity: cleavage of a bacteriophage phi80-induced RNA.

A qualitative scheme is presented that is directed towards the understanding of the differences in RNase P cleavage site specificity for these substrates.

Different pattern of codon recognition by mammalian mitochondrial tRNAs.

Analysis of an almost complete mammalian mitochondrial DNA sequence has identified 23 possible tRNA genes and we speculate here that these are sufficient to translate all the codons of the

Transfer ribonucleic acid biosynthesis. Substrate specificity of ribonuclease P.

The enzymic specificity of RNase P in vitro reflects the in vivo pathway for serine tRNA biosynthesis, where the C-C-A sequence is synthesized while the serineTRNA sequence is still a part of the large precursor RNA.