tRNA intergenic spacers reveal polymorphisms diagnostic for Xanthomonas albilineans.

  title={tRNA intergenic spacers reveal polymorphisms diagnostic for Xanthomonas albilineans.},
  author={Rhonda J. Honeycutt and Bruno W. S. Sobral and Michael McClelland},
  volume={141 ( Pt 12)},
A PCR-based detection system was developed for Xanthomonas albilineans, a pathogen of sugarcane, and other related xanthomonads, using the conserved sequence of two adjacent tRNA genes and the variable length and sequence of the spacer region between them. An appropriate region was identified as follows: tRNA genes with the same anticodon from a wide variety of bacteria were aligned and the most frequent base at each position was chosen to derive primers that would anneal to the gene in either… CONTINUE READING


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