tRNA-guanine transglycosylase from Escherichia coli: gross tRNA structural requirements for recognition.

@article{Curnow1993tRNAguanineTF,
  title={tRNA-guanine transglycosylase from Escherichia coli: gross tRNA structural requirements for recognition.},
  author={Alan W. Curnow and F L Kung and Keith A Koch and George A. Garcia},
  journal={Biochemistry},
  year={1993},
  volume={32 19},
  pages={5239-46}
}
tRNA-guanine transglycosylase (TGT) is the enzyme responsible for the post-transcriptional modification of specific tRNAs (those for Asn, Asp, His, and Tyr) with the hypermodified base, queuine. In Escherichia coli this enzyme catalyzes the exchange of guanine-34 in the anticodon with preQ1, which is subsequently further modified to queuine. There is evidence that such hypermodified tRNA molecules may play a role in the control of cell proliferation and differentiation. In order to perform… CONTINUE READING