siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis

  title={siRNAs compete with miRNAs for methylation by HEN1 in Arabidopsis},
  author={Bin Yu and Liu Bi and Jixian Zhai and Manu Agarwal and Shengben Li and Qingfa Wu and Shou-Wei Ding and Blake C. Meyers and Herv{\'e} Vaucheret and Xuemei Chen},
  booktitle={Nucleic acids research},
Plant microRNAs (miRNAs) and small interfering RNAs (siRNAs) bear a 2'-O-methyl group on the 3'-terminal nucleotide. This methyl group is post-synthetically added by the methyltransferase protein HEN1 and protects small RNAs from enzymatic activities that target the 3'-OH. A mutagenesis screen for suppressors of the partial loss-of-function hen1-2 allele in Arabidopsis identified second-site mutations that restore miRNA methylation. These mutations affect two subunits of the DNA-dependent RNA… CONTINUE READING
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