siRNA-mediated off-target gene silencing triggered by a 7 nt complementation

  title={siRNA-mediated off-target gene silencing triggered by a 7 nt complementation},
  author={Xiaoyu Lin and Xiaoan Ruan and Mark G. Anderson and Jeffrey A. McDowell and Paul E. Kroeger and Stephen W. Fesik and Yu Shen},
  journal={Nucleic Acids Research},
  pages={4527 - 4535}
A growing body of evidence suggests that siRNA could generate off-target effects through different mechanisms. However, the full impact of off-target gene regulation on phenotypic induction and accordingly on data interpretation in the context of large-scale siRNA library screen has not been reported. Here we report on off-target gene silencing effects observed in a large-scale knockdown experiment designed to identify novel regulators of the HIF-1 pathway. All of the three 'top hits' from our… CONTINUE READING
Highly Influential
This paper has highly influenced 12 other papers. REVIEW HIGHLY INFLUENTIAL CITATIONS


Publications citing this paper.
Showing 1-10 of 161 extracted citations

High Content Screening

Methods in Molecular Biology • 2018
View 10 Excerpts
Highly Influenced

New Algorithm for Analysis of Off-target Effects in siRNA Screens

View 5 Excerpts
Highly Influenced

Vigilance and validation: Keys to success in RNAi screening.

ACS chemical biology • 2011
View 15 Excerpts
Highly Influenced


Publications referenced by this paper.
Showing 1-10 of 24 references

An approach to genomewide screens of expressed small interfering RNAs in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America • 2004

Similar Papers

Loading similar papers…