siRNA-mediated off-target gene silencing triggered by a 7 nt complementation

@article{Lin2005siRNAmediatedOG,
  title={siRNA-mediated off-target gene silencing triggered by a 7 nt complementation},
  author={Xiaoyu Lin and Xiaoan Ruan and Mark G. Anderson and Jeffrey A. McDowell and Paul E. Kroeger and Stephen W. Fesik and Yu Shen},
  journal={Nucleic Acids Research},
  year={2005},
  volume={33},
  pages={4527 - 4535}
}
A growing body of evidence suggests that siRNA could generate off-target effects through different mechanisms. However, the full impact of off-target gene regulation on phenotypic induction and accordingly on data interpretation in the context of large-scale siRNA library screen has not been reported. Here we report on off-target gene silencing effects observed in a large-scale knockdown experiment designed to identify novel regulators of the HIF-1 pathway. All of the three 'top hits' from our… CONTINUE READING
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