siRNA cell arrays for high-content screening microscopy.

  title={siRNA cell arrays for high-content screening microscopy.},
  author={Holger Erfle and Jeremy C Simpson and Philippe I H Bastiaens and Rainer Pepperkok},
  volume={37 3},
          454-8, 460, 462
RNA interference (RNAi) is a recent advance that provides the possibility to reduce the expression of specific target genes in cultured mammalian cells with potential applications on a genome-wide scale. However, to achieve this, robust methodologies that allow automated and efficient delivery of small interfering RNAs (siRNAs) into living cultured cells and reliable quality control of siRNA function must be in place. Here we describe the production of cell arrays for reverse transfection of… CONTINUE READING
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