shRNA driven by Pol II/T7 dual-promoter system effectively induce cell-specific RNA interference in mammalian cells.

Abstract

Although Pol III promoters synthesize shRNA and elicit RNAi efficiently, however, a major limitation is that they are constitutively expressed in all cell types. To circumvent this problem, in the present study, we described a novel shRNA vector based on Pol II/T7 dual-promoter couple system: the transcription of shRNA under the control of T7 promoter is… (More)

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