shRNA Expression Constructs Designed Directly from siRNA Oligonucleotide Sequences

  title={shRNA Expression Constructs Designed Directly from siRNA Oligonucleotide Sequences},
  author={Tuva Bar{\o}y and Kirsten S{\o}rensen and Mona Mari Lindeberg and Eirik Frengen},
  journal={Molecular Biotechnology},
Gene silencing by RNA interference (RNAi) is a widely used approach for target-specific knockdown of gene expression. Induction of RNAi in mammalian cells can be achieved by introduction of synthetic small interfering RNA (siRNA) resulting in transient knockdown, or alternatively by stable expression of short hairpin RNA (shRNA). Several algorithms for efficient siRNA design exist, but recent reports have suggested that these cannot be directly used to design efficient shRNAs. In this study, 25… 

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