rep-PCR-Mediated Genomic Fingerprinting: A Rapid and Effective Method to Identify Clavibacter michiganensis.

@article{Louws1998repPCRMediatedGF,
  title={rep-PCR-Mediated Genomic Fingerprinting: A Rapid and Effective Method to Identify Clavibacter michiganensis.},
  author={Frank J. Louws and Jeremey D. Bell and C M Medina-Mora and Christine D. Smart and Dawn Opgenorth and Carol A. Ishimaru and Mary K. Hausbeck and Frans J de Bruijn and Dennis W. Fulbright},
  journal={Phytopathology},
  year={1998},
  volume={88 8},
  pages={862-8}
}
ABSTRACT The genomic DNA fingerprinting technique known as repetitive-sequence-based polymerase chain reaction (rep-PCR) was evaluated as a tool to differentiate subspecies of Clavibacter michiganensis, with special emphasis on C. michiganensis subsp. michiganensis, the pathogen responsible for bacterial canker of tomato. DNA primers (REP, ERIC, and BOX), corresponding to conserved repetitive element motifs in the genomes of diverse bacterial species, were used to generate genomic fingerprints… CONTINUE READING

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