qPCA: a scalable assay to measure the perturbation of protein-protein interactions in living cells.

@article{Freschi2013qPCAAS,
  title={qPCA: a scalable assay to measure the perturbation of protein-protein interactions in living cells.},
  author={Luca Freschi and Francisco Torres-Quiroz and Alexandre K. Dub{\'e} and Christian R. Landry},
  journal={Molecular bioSystems},
  year={2013},
  volume={9 1},
  pages={36-43}
}
One of the most important challenges in systems biology is to understand how cells respond to genetic and environmental perturbations. Here we show that the yeast DHFR-PCA, coupled with high-resolution growth profiling (DHFR-qPCA), is a straightforward assay to study the modulation of protein-protein interactions (PPIs) in vivo as a response to genetic, metabolic and drug perturbations. Using the canonical Protein Kinase A (PKA) pathway as a test system, we show that changes in PKA activity can… CONTINUE READING

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