piggyBac internal sequences are necessary for efficient transformation of target genomes.

@article{Li2005piggyBacIS,
  title={piggyBac internal sequences are necessary for efficient transformation of target genomes.},
  author={Xiang Li and Robert A. Harrell and Alfred M. Handler and T. R. Beam and Kevin M Hennessy and Malcolm J. Fraser},
  journal={Insect molecular biology},
  year={2005},
  volume={14 1},
  pages={17-30}
}
A previously reported piggyBac minimal sequence cartridge, which is capable of efficient transposition in embryo interplasmid transposition assays, failed to produce transformants at a significant frequency in Drosophila melanogaster compared with full-length or less extensive internal deletion constructs. We have re-examined the importance of these internal domain (ID) sequences for germline transformation using a PCR strategy that effectively adds increasing lengths of ID sequences to each… CONTINUE READING

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