piggyBac‐mediated germline transformation in the beetle Tribolium castaneum

@article{Lorenzen2003piggyBacmediatedGT,
  title={piggyBac‐mediated germline transformation in the beetle Tribolium castaneum},
  author={Marc{\'e} D. Lorenzen and Andreas J. Berghammer and S J Brown and Robin E. Denell and Martin Klingler and Richard W. Beeman},
  journal={Insect Molecular Biology},
  year={2003},
  volume={12}
}
The lepidopteran transposable element piggyBac can mediate germline insertions in at least four insect orders. It therefore shows promise as a broad‐spectrum transformation vector, but applications such as enhancer trapping and transposon‐tag mutagenesis are still lacking. We created, cloned, sequenced and genetically mapped a set of piggyBac insertions in the red flour beetle, Tribolium castaneum. Transpositions were precise, and specifically targeted the canonical TTAA recognition sequence… 
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