pac sites are indispensable for in vivo packaging of DNA by phage P22

  • H. Schmieger
  • Published 1984 in Molecular and General Genetics MGG

Abstract

F′ pro + plasmids were selected and used as donors to prepare P22 transducing phages. Two types of result were observed. pro + from type I donors cannot be packaged by wild-type P22 to yield transducing particles unless a prophage pac site is introduced into the plasmid. Transposon Tn10 also allows initiation of packaging. pro + from type II plasmids can be transduced with the same efficiency as pro + DNA on the chromosome, indicating that a chromosomal pac site was included when the F′ pro + was excised from the Hfr strain. The usefulness of type I plasmids as a test substrate for pac signals is discussed.

DOI: 10.1007/BF00332755

Cite this paper

@article{Schmieger1984pacSA, title={pac sites are indispensable for in vivo packaging of DNA by phage P22}, author={H. Schmieger}, journal={Molecular and General Genetics MGG}, year={1984}, volume={195}, pages={252-255} }