pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.

@article{Rther1982pUR2A,
  title={pUR 250 allows rapid chemical sequencing of both DNA strands of its inserts.},
  author={Ulrich U R{\"u}ther},
  journal={Nucleic acids research},
  year={1982},
  volume={10 19},
  pages={5765-72}
}
A multipurpose plasmid, pUR 250, has been constructed. It contains seven unique cloning sites (Hind III, Xba I, Sal I, Acc I, Hinc II, BamH I and EcoR I) in a small region of its lac Z gene part. Insertion of foreign DNA into the plasmid can be easily detected. Plasmid DNA isolated from a clone by a rapid method can be used to determine the DNA sequence of the insert from both ends directly without isolation of labeled fragment. 

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 47 extracted citations

Similar Papers

Loading similar papers…