pSEUDO, a genetic integration standard for Lactococcus lactis.

  title={pSEUDO, a genetic integration standard for Lactococcus lactis.},
  author={Jo{\~a}o Carlos Pizani Pinto and Araz Zeyniyev and Harma A. Karsens and H. F. Trip and Juke S. Lolkema and Oscar P. Kuipers and Jan Kok},
  journal={Applied and environmental microbiology},
  volume={77 18},
Plasmid pSEUDO and derivatives were used to show that llmg_pseudo_10 in Lactococcus lactis MG1363 and its homologous locus in L. lactis IL1403 are suitable for chromosomal integrations. L. lactis MG1363 and IL1403 nisin-induced controlled expression (NICE) system derivatives (JP9000 and IL9000) and two general stress reporter strains (NZ9000::PhrcA-GFP and NZ9000::PgroES-GFP) enabling in vivo noninvasive monitoring of cellular fitness were constructed. 
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