pK-matched running buffers for gel electrophoresis.

  title={pK-matched running buffers for gel electrophoresis.},
  author={Qiang Liu and Xiuqing Li and S. S. Sommer},
  journal={Analytical biochemistry},
  volume={270 1},
Electrophoresis through agarose and polyacrylamide-type gels is the standard method to separate, identify, and purify nucleic acids. Properties of electrophoresis buffers such as pH, ionic strength, and composition affect performance. The buffers in use contain a weak acid or weak base buffered by a compound with a dissimilar pK. Herein, three pK-matched buffers were developed, each containing two effective buffering components: one weak base and one weak acid which have similar pKa at 25… CONTINUE READING