pI-based fractionation of serum proteomes versus anion exchange after enhancement of low-abundance proteins by means of peptide libraries.

@article{Restuccia2009pIbasedFO,
  title={pI-based fractionation of serum proteomes versus anion exchange after enhancement of low-abundance proteins by means of peptide libraries.},
  author={Umberto Restuccia and Egisto Boschetti and Elisa Fasoli and Fr{\'e}d{\'e}ric Fortis and Luc Guerrier and Angela Bachi and Alexander V Kravchuk and Pier Giorgio Righetti},
  journal={Journal of proteomics},
  year={2009},
  volume={72 6},
  pages={1061-70}
}
The pre-treatment of biological extracts with the aim of detecting very low-abundance proteins generates complexity requiring a proper fractionation. Therefore the success of identifying all newly detectable species depends on the selected fractionation methods. In this context and starting from a human serum, where the dynamic concentration range was reduced by means of a preliminary treatment with a combinatorial hexapeptide ligand library, we fractionated the sample using a novel method… CONTINUE READING

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