pHUSH: a single vector system for conditional gene expression

  title={pHUSH: a single vector system for conditional gene expression},
  author={Daniel C. Gray and Klaus P. Hoeflich and Li Peng and Zhenyu Gu and Alvin Gogineni and Lesley J. Murray and Mike Eby and Noelyn M Kljavin and Somasekar Seshagiri and Mary J. Cole and David P. Davis},
  journal={BMC Biotechnology},
  pages={61 - 61}
BACKGROUND Conditional expression vectors have become a valuable research tool to avoid artefacts that may result from traditional gene expression studies. However, most systems require multiple plasmids that must be independently engineered into the target system, resulting in experimental delay and an increased potential for selection of a cell subpopulation that differs significantly from the parental line. We have therefore developed pHUSH, an inducible expression system that allows… CONTINUE READING
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