pH kinetic studies of bovine brain hexokinase.

@article{Solheim1980pHKS,
  title={pH kinetic studies of bovine brain hexokinase.},
  author={Lisa Solheim and Herbert J. Fromm},
  journal={Biochemistry},
  year={1980},
  volume={19 26},
  pages={6074-80}
}
The variation of kinetic parameters with pH was examined for bovine brain hexokinase with glucose and MgATP as substrates. The -log V1 and -log (V1/Km) profiles for both substrates were examined and seen to decrease below pH 6.5. All profiles asymptotically approached slopes of -1, indicating that the loss of activity in each instance was due to the protonation of a single group on the enzyme. Analysis of the data indicated two ionizable groups were involved in the reaction. One functions in… CONTINUE READING