pH-induced conformational changes in Clostridium difficile toxin B.

  title={pH-induced conformational changes in Clostridium difficile toxin B.},
  author={Maen Qa'Dan and Lea M Spyres and Jimmy Dale Ballard},
  journal={Infection and immunity},
  volume={68 5},
Toxin B from Clostridium difficile is a monoglucosylating toxin that targets substrates within the cytosol of mammalian cells. In this study, we investigated the impact of acidic pH on cytosolic entry and structural changes within toxin B. Bafilomycin A1 was used to block endosomal acidification and subsequent toxin B translocation. Cytopathic effects could be completely blocked by addition of bafilomycin A1 up to 20 min following toxin treatment. Furthermore, providing a low extracellular pH… CONTINUE READING

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