pH-induced alterations in the fusogenic spike protein of Semliki Forest virus

@article{Kielian1985pHinducedAI,
  title={pH-induced alterations in the fusogenic spike protein of Semliki Forest virus},
  author={Margaret Kielian and Ari Helenius},
  journal={The Journal of Cell Biology},
  year={1985},
  volume={101},
  pages={2284 - 2291}
}
The spike glycoproteins of Semliki Forest virus mediate membrane fusion between the viral envelope and cholesterol-containing target membranes under conditions of mildly acidic pH (pH less than 6.2). The fusion reaction is critical for the infectious cycle, catalyzing virus penetration from the acidic endosome compartment. To define the role of the viral spike glycoproteins in the fusion reaction, conformational changes in the spikes at acid pH were studied using protease digestion and binding… CONTINUE READING